Technical Data
C9097-40D1
Cytokeratin 7 (Cytokeratin-7, CK 7, CK7, CK-7, Keratin 55k Type II Cytoskeletal, Keratin 7, Keratin-7, K7, KRT7, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, K2C7, MGC3625, MGC129731, Sarcolectin, SCL, Type-II Keratin Kb7, Type II
Description:
Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.

Applications:
Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 10ul per test
Immunocytochemistry: 1:10 using isolated EVT cells fixed with 0.5% PFA at 4C for 15 minutes followed by acetone at -20C for 2 minutes.
Immunohistochemistry (Paraffin embedded sections): Protease treatment of paraffin sections is required.
Immunohistochemistry (Frozen): 1:10 used with placental tissue (7um) fixed with acetone for 1 hour.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Source: Tissue culture supernatant

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2b2Q1023Highly Purified
SizeStorageShippingSourceHost
100Tests-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Cytoskeletal proteins from cultured HeLa cells.
Purity:
Purified by ammonium sulfate precipitation followed by ion exchange chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes human Cytokeratin 7.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Osborn, M. et al. Lab. Invest. 55, 497-504 (1986). 2. Moll, R. et al Cell 31, 11-24 (1982). 3. Ramaekers, F. et al. Path. 11, 14-17 (1990). 4. Vojtesek, B. et al. Neoplasma 37, 333-42 (1989).