Technical Data
Cytokeratin, pan
Cytokeratin filaments consist of a complex family of approximately 20 different cytokeratin polypeptides with relative molecular weights of 40kD to 68kD. These intracellular intermediate filaments of 10nm in diameter provide structural integrity to epithelial cells. All the filament subunits share a general common fibrillar organization, consisting of a central alpha-helical rod that is flanked by N- and C-terminal domains at each end. Generally, low molecular weight keratins are expressed in the early stages of cell development, whereas high molecular weight keratins are expressed in maturing cells. This antibody may be useful in determining if a neoplasm originated from an epithelial or non-epithelial source, and is especially useful for the distinction of metastatic malignant tumors of epithelial origin.

Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:2000
Immunohistochemistry (Frozen): 1:100. Incubate for 1hr at RT.
Immunohistochemistry (Paraffin): 1:50. Incubate for >1hr at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanGuinea pig
Not Determined
Synthetic peptides corresponding to human keratins.
Supplied as a liquid, 0.09% sodium azide.
Recognizes most acidic (K1, K4, K8) and basic (K14) human cytokeratins (Mr 40,000-Mr 68,000 polypeptides). Tumors Specifically Detected: All types of epithelia and epithelium-derived neoplasms. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Akat, K., et al., Acta Neuropathol. 106: 337-347 (2003). 2. Boehnke, K., et al., Eur. J. Cell Biol. 86: 731-746 (2007).