		Technical Data
C9100-03	Cytomegalovirus (Biotin)

Description:
Cytomegalovirus is an enveloped double stranded DNA virus which in humans (HCMV) is commonly known as human herpesvirus 5 (HHV-5). Glycoprotein B (gB) is the main antigenic protein within the envelope of HCMV. It is required for HCMV mediated membrane fusion along with the gH/gL complex. The unprocessed precursor of HCMV gB is 906 amino acids in length, with a MW of ~100kD.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
1ml		4°C (-20°C Glycerol)	Blue Ice		Goat

Concentration:

4-5mg/ml

Immunogen:

Virions of Strain AD 169

Purity:

Purified

Form

Supplied as a liquid, 0.01M PBS, pH 7.2, 0.1% sodium azide

Specificity:

Recognizes glycoprotein B(gB). Does not react with HSV and VZV by indirect immunofluorescence. Negative vs HEp-2 cells and human fibroblasts by IFA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.