		Technical Data
C9100-21N	Cytomegalovirus Glycoprotein B, Late Neutralizing Antigen (CMV gB, CMV Glycoprotein GP55, HCMV, Human Herpesvirus 5, HHV-5, HHV5, UL55)

Description:
Glycoprotein B (gB) is the main antigenic protein within the envelope of HCMV. It is required for CMV mediated membrane fusion along with the gH/gL complex. The unprocessed precursor of HCMV gB is 906aa in length, with a MW of ~100kD.

Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: Use acetone-fixed CMV infected cells or frozen sections.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	10B2664	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice		Mouse

Concentration:

~1mg/ml

Immunogen:

Human CMV extracts.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes Cytomegalovirus Glycoprotein B, Late Neutralizing Antigen. Reacts with MRC-5 infected cells but not uninfected cells. This antibody has been found to be neutralizing.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.