Technical Data
C9127
Cytotoxic Cells, Nonspecific
Description:
Non-specific cytotoxic cells (NCCs) in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32kD membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Inhibition Assay. In cytotoxicity assays, this antibody is used under saturating conditions to inhibit target cell conjugation. Other applications not tested.

Recommended Dilution:
Western Blot: 10ug/ml; Detects a 32kD protein representing nonspecific cytotoxic cell receptor protein.
Inhibition Assay: Has been successfully used in cytotoxic inhibition studies with NCC/NK/LAK cells, panning, and ELISA procedures.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM4i269Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Purified NCC cells from catfish.
Purity:
Purified IgM
Form
Supplied in a liquid buffer with 1mg/ml BSA and 0.05% sodium azide.
Specificity:
Recognizes NCC. Crossreacts with Bovine, Canine, Equine, Porcine, Rat, Tilapia and Catfish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.