Technical Data
Custom Polyclonal Antibody Production, Rabbit
USDA-Registered Polyclonal Production
United States Biological is known for more than 250,000 antibodies offered in our catalog.

We maintain current USDA certification as a qualified animal research facility, and comply with the Animal Welfare Act.
We follow guidelines set forth by the National Institute of Health (NIH) and have established an Institutional Animal Care and Use Committee (IACUC).

Antibody Program:
A typical standard protocol includes animal purchase, housing and care, all immunizations, bleeds and exsanguinations as per our standard protocol formats. Your project may require different specifications and, therefore, prices will vary. Our costs are quite reasonable. Although we will conduct single animal programs, the additional costs to add a second animal can produce a significantly greater yield and likelihood for success.

Standard 2 Rabbit Protocol:
Standard protocol pricing includes animal purchase, housing and care up to 49 days, all immunizations, bleeds and exsanguinations as per the following schedule:

Pre-Peptide Consultation if necessary

Required, but not included in price:
Peptide Production
Peptide Conjugation

Day 0 Pre-immunization bleed
Antigen Injection (s.c and i.m; CFA; 200ug)
Day 14 Antigen Injection (IFA; 100ug)
Day 28 Antigen Injection (IFA; 100ug)
Day 35 First Bleed (20-25ml/rabbit)
Day 42 Antigen Injection (IFA; 100ug)
Day 49 Final Production Bleed (~40-50ml/rabbit)
Return of antisera and peptides

Continuation (extra cost):
Day 63 Antigen Injection (IFA; 100ug)
Day 77 Third Bleed (20-25ml/rabbit)

Antigen Requirements:
Per Animal Minimum Recommended
Chickens 250ug 1mg
Rabbits 500ug 2mg
Goats 2.5mg 5mg
Sheep 2.5mg 5mg

The amount of antigen required varies from a minimum as shown for the standard protocol to a maximum when additional injections might be needed to boost antibody production or to continue a maintenance program longer than the stated time. Affinity columns will also require additional antigen.

Antibody Quotation Worksheet:
For a quotation, please supply the following information:

1. Antigen name or peptide sequence

2. Type of protein
o Natural
o Recombinant
o Synthetic
o Purity ___%
o MW. ___

3. Conjugation
o Ovalbumin
o Thyroglobulin

4. Number of animals required

5. Immunization protocol
o Customer Supplied
o United States Biological Supplied

Important Note:
Standard Project Termination:
All projects will automatically terminate 1 week after the expiration of the standard protocol unless a written request is made for the extension and additional charges are paid @$195/rabbit/month. This can be in the form of a Purchase Order. Please communicate with us in writing fax/email to avoid any confusion and make sure instructions are properly followed.
1 Rabbit-20CBlue Ice
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Webb, G.D. et al., (2008) J. Pharmacology and Exp. Pharmacology 324:876-882. 2. Han, M.-S. et al., (2004) Proc. Natl., Acad. Sci. 101: 1093-1098.