Technical Data
CS010-010
Custom Polyclonal Antibody Production, Rabbit
1 Rabbit
2 Rabbits
3 Rabbits
Custom Services Storage: -20CShipping: Blue Ice
USDA-Registered Polyclonal Production
United States Biological is known for more than 250,000 antibodies offered in our catalog.

We maintain current USDA certification as a qualified animal research facility, and comply with the Animal Welfare Act.
We follow guidelines set forth by the National Institute of Health (NIH) and have established an Institutional Animal Care and Use Committee (IACUC).

Antibody Program:
A typical standard protocol includes animal purchase, housing and care, all immunizations, bleeds and exsanguinations as per our standard protocol formats. Your project may require different specifications and, therefore, prices will vary. Our costs are quite reasonable. Although we will conduct single animal programs, the additional costs to add a second animal can produce a significantly greater yield and likelihood for success.

Standard 2 Rabbit Protocol:
Standard protocol pricing includes animal purchase, housing and care up to 49 days, all immunizations, bleeds and exsanguinations as per the following schedule:

Pre-Peptide Consultation if necessary

Required, but not included in price:
Peptide Production
Peptide Conjugation

Day 0 Pre-immunization bleed
Antigen Injection (s.c and i.m; CFA; 200ug)
Day 14 Antigen Injection (IFA; 100ug)
Day 28 Antigen Injection (IFA; 100ug)
Day 35 First Bleed (20-25ml/rabbit)
ELISA
Day 42 Antigen Injection (IFA; 100ug)
Day 49 Final Production Bleed (~40-50ml/rabbit)
ELISA
Return of antisera and peptides

Continuation (extra cost):
Day 63 Antigen Injection (IFA; 100ug)
Day 77 Third Bleed (20-25ml/rabbit)

Antigen Requirements:
Per Animal Minimum Recommended
Chickens 250ug 1mg
Rabbits 500ug 2mg
Goats 2.5mg 5mg
Sheep 2.5mg 5mg

The amount of antigen required varies from a minimum as shown for the standard protocol to a maximum when additional injections might be needed to boost antibody production or to continue a maintenance program longer than the stated time. Affinity columns will also require additional antigen.

Antibody Quotation Worksheet:
For a quotation, please supply the following information:

1. Antigen name or peptide sequence

2. Type of protein
o Natural
o Recombinant
o Synthetic
o Purity ___%
o MW. ___

3. Conjugation
o BSA
o KLH
o Ovalbumin
o Thyroglobulin

4. Number of animals required

5. Immunization protocol
o Customer Supplied
o United States Biological Supplied

Important Note:
Standard Project Termination:
All projects will automatically terminate 1 week after the expiration of the standard protocol unless a written request is made for the extension and additional charges are paid @$195/rabbit/month. This can be in the form of a Purchase Order. Please communicate with us in writing fax/email to avoid any confusion and make sure instructions are properly followed.
Optional Antibody Services (by quotation):

Purification:
Rabbit polyclonal antibodies can be affinity purified. This is best suited for when serum alone often has too much non-specific interference.

ELISA:
Each antibodies (from 2 rabbits) will be tested in a standard ELISA format for titer.

Western Blot: Purified antibody is tested in Western Blot against a variety of normal tissue, tumor tissue, and cell line lysates (see below). Just select which lysates you would like to have run. A full panel of 14 lysates of your choosing is provided, which yields a more comprehensive expression pattern and allows for testing in human, mouse, rat tissue and cell lines. Scans of the blot in an electronic image are supplied.

Immunohistochemistry:
Purified antibodies are suitable for use in Immunohistochemistry. We have a comprehensive variety of normal and tumor tissues, and slides. This service includes testing for five tissues. Electronic images are provided.

Lysates may be supplied by group only. Selection may change:
293 whole cell lysate
3T3 whole cell lysate
A431 whole cell lysate
A549 whole cell lysate
Caco-2 whole cell lysate
Daudi whole cell lysate
HeLa whole cell lysate
HepG2 whole cell lysate
HL60 whole cell lysate
HT-29 whole cell lysate
Jurkat whole cell lysate
K562 whole cell lysate
MCF7 whole cell lysate
MDA-MB-361 whole cell lysate
MOLT4 whole cell lysate
PC-3 whole cell lysate
Raji whole cell lysate
Ramos whole cell lysate
SK-N-SH whole cell lysate
SW1353 whole cell lysate
SW480 whole cell lysate
T24 whole cell lysate
THP-1 whole cell lysate
U937 whole cell lysate
WI-38 whole cell lysate

Human Tissue Lysate
Human brain tissue lysate
Human heart tissue lysate
Human kidney tissue lysate
Human liver tissue lysate
Human lung tissue lysate
Human pancreas tissue lysate
Human small intestine tissue lysate
Human spleen tissue lysate

Human Tumor Tissue Lysate
Human brain tumor lysate
Human breast tumor lysate
Human colon normal tissue lysate
Human colon tumor tissue lysate
Human kidney tumor lysate
Human liver normal tissue lysate
Human liver tumor tissue lysate
Human lung tumor lysate

Mouse Tissue Lysate
Mouse adipose tissue lysate
Mouse adrenal tissue lysate
Mouse bladder tissue lysate
Mouse brain tissue lysate
Mouse cerebellum tissue lysate
Mouse cerebrum tissue lysate
Mouse colon tissue lysate
Mouse heart tissue lysate
Mouse kidney tissue lysate
Mouse liver tissue lysate
Mouse lung tissue lysate
Mouse pancreas tissue lysate
Mouse skeletal muscle tissue lysate
Mouse skin tissue lysate
Mouse small intestine tissue lysate
Mouse spleen tissue lysate
Mouse stomach tissue lysate
Mouse testis tissue lysate
Mouse thymus tissue lysate
Mouse whole eye tissue lysate

Rat Tissue Lysate
Rat adrenal tissue lysate
Rat bladder tissue lysate
Rat brain tissue lysate
Rat cerebellum tissue lysate
Rat cerebrum tissue lysate
Rat colon tissue lysate
Rat eye tissue lysate
Rat heart tissue lysate
Rat kidney tissue lysate
Rat liver tissue lysate
Rat lung tissue lysate
Rat pancreas tissue lysate
Rat skeletal muscle tissue lysate
Rat skin tissue lysate
Rat small intestine tissue lysate
Rat spleen tissue lysate
Rat stomach tissue lysate
Rat testis tissue lysate
Rat thymus tissue lysate

IACUC
Antibody programs are produced in a facility which maintains current USDA certification as a qualified animal research facility, and complies with the Animal Welfare Act. Guidelines are set forth by the National Institute of Health (NIH). An established Institutional Animal Care and Use Commitee (IACUC) reviews all production protocols prior, during and after all client programs.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
US Biological application reference: Webb, G.D. et al., (2008) J. Pharmacology and Exp. Pharmacology 324:876-882. 2. Han, M.-S. et al., (2004) Proc. Natl., Acad. Sci. 101: 1093-1098.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.