Technical Data

Media

Defined Nutrient Powder Modified SAAMB (Powder)

CS050M016

Storage RT/4°C Shipping RT

Components shown as mg/liter


Inorganic Salts:
Ammonium Sulfate	428.55
Calcium Chloride	5.57
Ferric Chloride•6H2O	2.7
Magnesium Chloride	95.35
Manganese Sulfate•H2O	0.273
Potassium Chloride	100
Sodium Chloride	1000
Zinc Sulfate•7H2O	0.8

Amino Acids:
L-Arginine HCl	1270
L-Asparagine	999.68
L-Aspartic acid	20
L-Cysteine	1000.12
L-Cystine•Na2•H2O	301.86
L-Glutamic acid	20
L-Glutamine	2520
Glycine	500
L-Histidine	310.1
L-Isoleucine	520
L-Leucine	520
L-Lysine•HCI	724.65
L-Methionine	150
L-Phenylalanine	320
L-Proline	1000
L-Serine	30
L-Threonine	480
L-Tryptophan	100
L-Tyrosine•2Na•2H2O	519
L-Valine	460

Vitamins:
p-aminobenzoic acid	1
Biotin	0.5
Choline Chloride	25
Folic Acid	5
i-Inositol	25
Nicotinamide	5
D-Pantothenic Acid•Ca	25
Pyridoxal•HCI	5
Riboflavin	5
Thiamine•HCI	5

Other:
Adenine	25
Sodium Pyruvate	1000
Uracil	25

Total:	14.5g/liter

Appearance:
Off-white to pale yellow, homogenous, free flowing powder

Solubility:
Pale yellow, clear, complete

pH:
7.0 ± 1.0

Endotoxin:
1EU/ml

Directions per Liter: Dissolve 14.5g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

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Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.