Technical Data
Media
Defined Nutrient Powder Modified SAAMB (Powder)
CS050M016
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Ammonium Sulfate428.55
Calcium Chloride5.57
Ferric Chloride•6H2O2.7
Magnesium Chloride95.35
Manganese Sulfate•H2O0.273
Potassium Chloride100
Sodium Chloride1000
Zinc Sulfate•7H2O0.8
Amino Acids:
L-Arginine HCl1270
L-Asparagine999.68
L-Aspartic acid20
L-Cysteine1000.12
L-Cystine•Na2•H2O301.86
L-Glutamic acid20
L-Glutamine2520
Glycine500
L-Histidine310.1
L-Isoleucine520
L-Leucine520
L-Lysine•HCI724.65
L-Methionine150
L-Phenylalanine320
L-Proline1000
L-Serine30
L-Threonine480
L-Tryptophan100
L-Tyrosine•2Na•2H2O519
L-Valine460
Vitamins:
p-aminobenzoic acid1
Biotin0.5
Choline Chloride25
Folic Acid5
i-Inositol25
Nicotinamide5
D-Pantothenic Acid•Ca25
Pyridoxal•HCI5
Riboflavin5
Thiamine•HCI5
Other:
Adenine25
Sodium Pyruvate1000
Uracil25
Total:14.5g/liter
Appearance:
Off-white to pale yellow, homogenous, free flowing powder

Solubility:
Pale yellow, clear, complete

pH:
7.0 ± 1.0

Endotoxin:
1EU/ml


Directions per Liter: Dissolve 14.5g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

:


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.