Technical Data
CS090-05
Custom Protein Expression, Baculovirus
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Baculovirus Expression:
United States Biological offers a baculovirus recombinant protein expression service. Our high-level baculovirus expression system includes fast turn-around time, online progress tracking, and competitive pricing.

Breakdown of Standard Expression:

Phase I:
* Amplification/isolation of the gene of interest out of a customer-supplied vector and subcloning of it into a transfer vector with His-tag.
* Sequencing confirmation of insert.
* Transformation of E. coli with baculoviral DNA and gene of interest.
* Culture of E. coli and purification using mini-prep to produce bacmid (recombinant viral DNA).
* Transfection of sf9 insect cells with bacmid.
* Plaque purification and viral titer assay.
* Small-scale culture to test expression level.
Estimated time: 4-6 weeks

Phase II:
* Virus stock preparation
* Large-scale culture and infection of insect cells for protein expression.
* Purification from 1 liter of infected insect cell using affinity column of Ni-NTA or other column.
Estimated time: 2 weeks
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.