Technical Data
D0900-19A
DAB2 (Disabled Homolog 2, Mitogen-responsive Phosphoprotein (Drosophila), DOC2, DOC-2)
Description:
Dab2 (Disabled homolog 2, Diabled-2) is a mammalian structural homolog of Drosophila Disabled protein and a mitogen-responsive phosphoprotein (1). As a component of the CSF1 signal transduction pathway, Dab2 is normally expressed in ovarian epithelia cells. However, in ovarian carcinoma, Dab2 expression is either lost or down-regulated. Therefore, Dab2 is speculated to be a negative regulator of growth and its down-regulation considered to play critical role in ovarian carcinogenesis (2).

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000-10,000
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:20
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E259Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues near C-terminal of human Dab2.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Specificity:
Recognizes human DAB2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Morris SM., et al. EMBO J. 21:1555–1564, 2002. 2. Sheng Z., et al. Oncogene 19:4847–4854, 2000.
3. Mok SC., et al. Gynecol. Oncol. 52:247–252. 1994.