Technical Data
D1055-01B
DACT3 (Dapper Homolog 3, MGC15476, RRR1, Thymus Expressed Gene 3-like)
Description:
DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:125
Western Blot: 1:125-1:250
Optimal dilutions to be determined by the researcher.

Hybridoma:
F0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k9G464Affinity Purified
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant human DACT3, expressed in E. coli.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human DACT3 (89-202aa) at ~64kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jiang X, et al., (2008) Cancer Cell. 13(6):52941 2. Fisher DA, et al., (2006) Dev Dyn. 235(9):262030