		Technical Data
D1075-01R	DARPP32 (Dopamine and cAMP-regulated Phosphoprotein DARPP32, DARPP 32, DARPP-32, Dopamine- and cAMP-regulated Neuronal Phosphoprotein, FLJ20940, Neuronal Phosphoprotein DARPP-32, Protein Phosphatase 1 Regulatory (Inhibitor) Subunit 1B, PPP1R1B)

Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Rat	Rabbit

Concentration:

As reported

Immunogen:

Peptide corresponding to the N-terminal region of rat DARPP-32, conjugated to KLH.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 10mM HEPES, pH7.5, 0.09% sodium azide, 0.01% BSA, 50% glycerol.

Specificity:

Recognizes rat DARPP-32. Species sequence homology: zebrafish, mouse, dog, xenopus, bovine, chicken, human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Bibb, J. A. et al. (1999) Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons. Nature 402:669-671. 2. Fienberg, A. et al. (1998) DARPP-32: regulator of the efficacy of dopaminergic neurotransmission. Science 281:838-842.