Technical Data
D1075-01R
DARPP32 (Dopamine and cAMP-regulated Phosphoprotein DARPP32, DARPP 32, DARPP-32, Dopamine- and cAMP-regulated Neuronal Phosphoprotein, FLJ20940, Neuronal Phosphoprotein DARPP-32, Protein Phosphatase 1 Regulatory (Inhibitor) Subunit 1B, PPP1R1B)
Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
Not determined
Immunogen:
Peptide corresponding to the N-terminal region of rat DARPP-32, conjugated to KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH7.5, 0.09% sodium azide, 0.01% BSA, 50% glycerol.
Specificity:
Recognizes rat DARPP-32. Species sequence homology: zebrafish, mouse, dog, xenopus, bovine, chicken, human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bibb, J. A. et al. (1999) Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons. Nature 402:669-671. 2. Fienberg, A. et al. (1998) DARPP-32: regulator of the efficacy of dopaminergic neurotransmission. Science 281:838-842.