Technical Data
DARPP32 (Dopamine and cAMP-regulated Phosphoprotein DARPP32, DARPP 32, DARPP-32, Dopamine- and cAMP-regulated Neuronal Phosphoprotein, FLJ20940, Neuronal Phosphoprotein DARPP-32, Protein Phosphatase 1 Regulatory (Inhibitor) Subunit 1B, PPP1R1B)
Dopamine- and cAMP-regulated neuronal phosphoprotein (DARP-32) is a cytoplasmic member of the protein phosphatase inhibitor 1 family. DARP-32 is a multifunctional protein kinase and protein phosphatase regulator, depending on the site of its activation. It possesses at least four sites that can be phosphorylated. Phosphorylation by PKA at Thr34 stimulates DARP-32 to act as a protein-phosphatase-1 (PP-1) inhibitor. However, phosphorylation at Thr75 will inhibit Thr34 phosphorylation. This selective, bidirectional regulation allows DARPP-32 to have a wide range of influence on downstream physiological effectors such as: ion pumps, neurotransmitter receptors, voltage-gate ion channels, and transcriptional factors. Studies suggest that it may also have a role in the Circadian System photic pathway. Overexpression of DARPP-32 has been linked with antiapoptotic mechanisms of cancer cells and has been observed in breast, colon, esophagus, gastric, and prostate cancer tissues.

Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1ug/ml detects DARPP-32 on 10ug of rat brain tissue lysate.
Immunoprecipitation: 10ug immunoprecipitates DARPP-32 from rat brain RIPA lysate.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceMouseRabbit
Linear peptide corresponding to mouse DARPP-32 near the N-terminus (KLH). Cellular Localization: cytoplasm
Purified by affinity chromatography.
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Recognizes mouse DARPP-32. Species crossreactivity: rat. Species sequence homology: canine, Rhesus Macaque.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.