		Technical Data
D1075-02	DARPP32, phosphorylated (Thr75) (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)

Description:
DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum. It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity. Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1. DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological and behavioral phenotype.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not Determined

Immunogen:

Synthetic phosphopeptide corresponding to residues surrounding Thr75 of human DARPP32.

Purity:

Purified by Protein A and immunoaffinity chromatography.

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Specificity:

Recognizes endogenous levels of DARPP32 at ~32kD only when phosphorylated at threonine 75. Does not crossreact with DARPP32 phosphorylated at Thr34. Species Crossreactivity: mouse and rat. Species Sequence Homology: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Nishi, A., et al., J. Neurosci. 17: 8147-8155 (1997). 2. Bibb, J.A., et al., Nature 402: 669-671 (1999). 3. Fienberg, A.A., et al., Science 281: 838-842 (1998).