Technical Data
D1075-02C
DARPP32, NT (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)
Description:
The 32kD dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated(1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1(2,3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5 (Cdk5), DARPP-32 is converted into an inhibitor of PKA(1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers(4).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000-1:50,000
Immunoprecipitation: 1:50
Immunohistochemistry: 1:250-1:500
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot: Rat brain lysate
Immunohistochemistry: Human breast adenocarcinoma (Paraffin-embedded)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k146Supernatant
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues near the N-terminal of human DARPP-32
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human DARPP-32. Species Crossreactivity: Mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bibb, et al., Nature 402: 669-671 (1999). 2. Greengard, P., et al., Neuron 23: 435-447 (1999). 3. Hemmings, H.C. Jr., et al., Nature 310: 503-505 (1984). 4. El-Rifai, W., et al., Cancer Res. 62: 4061-4064 (2002).