Technical Data
D1075-02C
DARPP32, NT (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)
Description:
The 32kD dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated(1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1(2,3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5 (Cdk5), DARPP-32 is converted into an inhibitor of PKA(1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers(4).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (frozen and paraffin), Immunocytochemistry, Immunofluorescence and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000-1:50,000. Detects a band of ~32kD.
Immunoprecipitation: 1:50
Immunohistochemistry (frozen); 1:7,500
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot: Rat brain lysate
Immunohistochemistry: Human breast adenocarcinoma (Paraffin-embedded)

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgGEP720YSupernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues near the N-terminal of human DARPP-32
Purity:
Supernatant
Form
Supplied as a liquid in PBS containing 0.01% sodium azide, 0.05% BSA and 50% glycerol.
Specificity:
Recognizes human DARPP-32. Species Crossreactivity: mouse, porcine and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bibb, et al., Nature 402: 669-671 (1999). 2. Greengard, P., et al., Neuron 23: 435-447 (1999). 3. Hemmings, H.C. Jr., et al., Nature 310: 503-505 (1984). 4. El-Rifai, W., et al., Cancer Res. 62: 4061-4064 (2002).