Technical Data
D1075-02E
DARPP32, phosphorylated (Thr34) (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)
Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr34 of human DARPP-32
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 150mM sodium chloride, 100ug BSA and 50% glycerol.
Specificity:
Detects endogenous levels of DARPP-32 only when phosphorylated at threonine 34. Does not crossreact with DARPP-32 phosphorylated at Thr75.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.