Technical Data
DARPP32, phosphorylated (Thr34) (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)
DARPP-32, a dopamine (DA) and cAMP-regulated ~32kD phosphoprotein that is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia,1 inhibits protein phosphatase I when it is phosphory on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75, the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission2. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and ProzacŪ.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at -20°C for short-term only. For long-term storage, store at -70°C. Aliquots are stable for at least 12 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ul-70°CDry IceRabbit
10 mini-blots/100ul
Synthetic peptide derived from amino acids surrounding the phosphorylated Thr34 residue of DARPP-32.
Purified by immunoaffinity chromatography.
Supplied as a liquid in 10mM Hepes, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
Reacts with human, mouse, and rat DARPP-32 proteins phosphorylated at Thr34. On Western blots, it identifies the target band at ~32 kD. Reactivity has been confirmed with rat caudate slices incubated in the presence of forskolin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Svenningsson P, et al. Science 302:1412-1415, 2003.
2. Nish A, et al. J Neurochem 81(4):832-841, 2002.