Technical Data
D1075-04
DARPP32, phosphorylated (Thr75) (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)
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Technical Data |
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D1075-04 |
DARPP32, phosphorylated (Thr75) (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B) |
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Description: DARPP-32 is a dopamine- and cAMP-regulated phosphoprotein, which is principally expressed in striatal medium spiny neurons, and is thought to play a critical role in the regulation of dopaminergic neurotransmission. DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation. Phosphorylation at threonine 34 converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1). Alternatively, phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA) . Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, detects a band of approximately 32kD in rat brain lysates prepared from caudate slices that had been incubated in okadaic acid. Optimal dilution determined by the researcher. Recommended Secondary Reagents: I1904-40J: IgG, Fc (HRP) Pab Gt xRb Sheep anti rabbit IgG:HRP Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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