Technical Data
D1075-04
DARPP32, phosphorylated (Thr75) (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)
Description:
DARPP-32 is a dopamine- and cAMP-regulated phosphoprotein, which is principally expressed in striatal medium spiny neurons, and is thought to play a critical role in the regulation of dopaminergic neurotransmission. DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation. Phosphorylation at threonine 34 converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1). Alternatively, phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA) .

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000, detects a band of approximately 32kD in rat brain lysates prepared from caudate slices that had been incubated in okadaic acid.
Optimal dilution determined by the researcher.

Recommended Secondary Reagents:
I1904-40J: IgG, Fc (HRP) Pab Gt xRb
Sheep anti rabbit IgG:HRP

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr 75.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 0.01% BSA, 0.09% sodium azide, 50% glycerol.
Specificity:
Recognizes rat DARPP-32, also known as protein phosphatase 1 regulatory subunit 1B (PP1R1B), when phosphorylated at threonine 75. Species Crossreactivity: Human, mouse, bovine, canine, chicken and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.