Technical Data
D1075-05A
DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)
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Technical Data |
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D1075-05A |
DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B) |
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Description: Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32kD (DARPP-32), also known as PPP1R1B, is a 23kD protein that anomalously migrates at about 32-35kD on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Immunohistochemistry (Paraffin): 8-25ug/ml detects DARPP-32 in rat corpus striatum tissue. Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived recombinant human DARPP-32. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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