Technical Data
D1075-05B
DARPP32, phosphorylated (Thr75) (Protein Phosphatase 1 Regulatory Subunit 1B, DARPP-32, Dopamine- and cAMP-regulated Neuronal Phosphoprotein, PPP1R1B)
Description:
DARPP-32 is a dopamine (DA) and cAMP-regulated 32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:100-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic phosphopeptide corresponding to human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human DARPP32 when phosphorylated at Thr75. Species Cross-Reactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.