Technical Data
D1075-07
DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)
Description:
DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at threonine 34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at threonine 75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological and behavioral phenotype (3).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:100 using citrate/TBST for antigen retrieval.
Immunofluorescence (Frozen): 1:400
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
MabIgG7H17Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues surrounding Glu160 of human DARPP-32 (KLH).
Purity:
Supernatant
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Reccognizes endogenous levels of total DARPP-32 protein. Species Crossreactivity: mouse, rat. Species sequence homology: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nishi, A. et al. (1997) J. Neurosci. 17, 8147-8155. 2. Bibb, J.A. et al. (1999) Nature 402, 669-671.
3. Fienberg, A.A. et al. (1998) Science 281, 838-842.