Technical Data
D1560-01C
DDAH2 (N(G),N(G)-dimethylarginine Dimethylaminohydrolase 2, DDAH-2, Dimethylarginine Dimethylaminohydrolase 2, DDAHII, Dimethylargininase-2, Protein G6a, S-phase Protein, DDAH, G6A, NG30)
Description:
DDAH2 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Overexpression of DDAH2 has also been linked to increased VEGF mRNA expression and enhanced tube formation by cells grown in a 3-dimensional medium.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse Heart extracts, Human Heart extract

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to SSLCLVLSTRPHS, from the C-terminus of the protein sequence according to NP_039268.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes DDAH2. Species Crossreactivity: human and mouse. Species sequence homology: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Leiper JM, Santa Maria J, Chubb A, MacAllister RJ, Charles IG, Whitley GS, Vallance P. Identification of two human dimethylarginine dimethylaminohydrolases with distinct tissue distributions and homology with microbial arginine deiminases. Biochem J 1999 Oct 1;343 Pt 1:209-14 PMID: 10493931.