Technical Data
D1649-03G
DDR1 (Discoidin Domain Receptor Tyrosine Kinase 1, Epithelial Discoidin Domain-containing Receptor 1, Epithelial Discoidin Domain Receptor 1, EDDR1, CD167 Antigen-like Family Member A, CD167a, CD167, Cell Adhesion Kinase, CAK, DDR, Discoidin Receptor Tyro
Description:
The discoidin domain receptors (DDRs) are receptor tyrosine kinases with a discoidin homology
repeat in their extracellular domains, activated by binding to extracellular matrix collagens. So far, two mammalian DDRs have been identified: DDR1 and DDR2 (1). They are widely expressed in human tissues and may have roles in smooth muscle cell-mediated collagen remodeling (2). Aberrant expression and signaling of DDRs have been implicated in human disease related to increased matrix degradation and remodeling, such as cardiovascular disease, liver fibrosis,
and tumor invasion (1).

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IF-IC): 1:800
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Immunohistochemistry (FFPE): 1:6400 using citrate buffer retrieval.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG11C1024Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues surrounding Pro600 of human DDR1.
Purity:
Purified
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogeneous levels of total human DDR1. Species Crossreactivity: monkey, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Vogel, W. (1999) FASEB J. 13 Suppl S77-S82. (2) Ferri, N. et al. (2004) Am. J. Pathol. 164, 1575-1585.