Technical Data
DDX3 (DEAD Box Protein 3 X-chromosomal, DBX, Helicase-Like Protein 2, HLP2)
DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it
from other RNA helicases (1,2). DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome (3). DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation (4). It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma (5). Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports
the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm (6).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ugml
Optimal dilutions to be determined by the researcher.

Positive Control:
HepG2 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
As reported
Synthetic peptide DDX3 16 aa near the center of human DDX3 (Genbank accession No. AAC34298).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Recognizes human DDX3. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Linder P, Lasko PF, Ashburner M, et al. Nature 1989; 337:121-2. 2. Park SH, Lee SG, Kim Y, et al. Cytogenet. Cell Genet. 1998; 81:178-9. 3. Lahn BT and Page DC. Science 1997; 278:675-80. 4. Abdelhaleem M. Clin. Biochem. 2005; 38:499-503. 5. Chang PC, Chi CW, Chau GY, et al. Oncogene 2006; 25:1991-2003. 6. Yedavalli VS, Neuveut C, Chi YH, et al. Cell 2004; 119:381-92.