Technical Data
D1660-20E
DDX3, CT (ATP-dependent RNA Helicase DDX3X, DEAD Box Protein 3, X-Chromosomal, Helicase-like Protein 2, HLP2, DEAD Box, X Isoform, DDX3X, DBX)
Description:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3 is a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. The DDX3 gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the C-terminal region of human DDX3 (KLH).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human DDX3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ariumi,Y., J. Virol. 81(24), 13922-13926 (2007). 2. Hogbom,M., J. Mol. Biol. 372(1), 150-159 (2007). 3. Franca,R., Proteins 67(4), 1128-1137 (2007). 4. DDX3, Cancer Res. 66(13), 6579-6588 (2006).