Technical Data
D1660-20J
DDX3, ID (ATP-dependent RNA Helicase DDX3X, DEAD Box Protein 3, X-Chromosomal, Helicase-like Protein 2, HLP2, DEAD Box, X Isoform, DDX3X, DBX)
Description:
DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.

Applications:
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HepG2 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to 16aa from near the internal sequence of human DDX3.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human DDX3. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Linder, P. et al. Nature 337, 121 (1989). 2. Park, SH. et al. Cytogenet. Cell Genet. 81, 178 (1998). 3. Lahn, BT. et al. Science 278, 675 (1997). 4. Abdelhaleem, M. et al. Clin. Biochem. 38, 499 (2005). 5. Chang, PC. et al. Oncogene 25, 1991 (2006). 6. Yedavalli, VS. et al. Cell 119, 381 (2004).