Technical Data
D1660-22
DDX3, NT (DEAD Box Protein 3 X-chromosomal, DBX, Helicase-Like Protein 2, HLP2)
Description:
Description:
DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it
from other RNA helicases (1,2). DDX3 is localized to the
X chromosome and has a highly conserved functional
homolog (DBY) on the Y chromosome (3). DDX3 is
thought to be involved in RNA splicing, RNA transport, and
translation initiation (4). It has also been shown to be
involved in cell growth control and is deregulated in
hepatitis virus-associated hepatocellular carcinoma (5).
Recent experiments suppressing DDX3 expression
blocked HIV-1 RNA export from the nucleus, suggesting
that DDX3 functions as a shuttling protein that transports
the HIV-1 protein Rev and its cofactor CRM1 from the
nucleus to the cytoplasm (6).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml.
Optimal dilutions to be determined by the researcher.

Postive control:
HepG2 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide 16 aa, near the amino terminus of human DDX3 (Genbank accession No. AAC34298).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes human DDX3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Linder P, Lasko PF, Ashburner M, et al. Nature 1989; 337:121-2. 2. Park SH, Lee SG, Kim Y, et al. Cytogenet. Cell Genet. 1998; 81:178-9. 3. Lahn BT and Page DC. Science 1997; 278:675-80.
4. Abdelhaleem M. Clin. Biochem. 2005; 38:499-503. 5. Chang PC, Chi CW, Chau GY, et al. Oncogene 2006; 25:1991-2003. 6. Yedavalli VS, Neuveut C, Chi YH, et al. Cell 2004; 119:381-92.