Technical Data
D1660-22D
DDX3 (DEAD Box Protein 3 X-chromosomal, DBX, Helicase-Like Protein 2, HLP2)
Description:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. Alternative splicing of this gene generates 2 transcript variants, which are divergent in the 3' UTR.

Applications:
Suitable for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.04-0.1ug/ml
Immunoprecipitation: 2-5ug/mg lysate
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cells

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
The epitope that this antibody recognizes maps to a region between residues 600 and the C-terminus (residue 661) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 using the numbering given in entry NP_001347.2 (GeneID 1654).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
Specificity:
Recognizes human DDX3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.