Technical Data
D1660-25P
DDX3Y (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3 Y-linked, DBY) (Azide Free)
Description:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it has a homolog on the X chromosome. The gene mutation causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Alternatively spliced variants, encoding the same protein, have been identified.

Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1.5ug/ml
ELISA: 1ug/ml-3ng/ml
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110B2680Affinity Purified
SizeStorageShippingSourceHost
100ulBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Recombinant protein corresponding to a portion of amino acids within aa1-81 of human DDX3Y.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
Specificity:
Recognizes human DDX3Y.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.