Technical Data
DDX4 (Probable ATP-dependent RNA Helicase DDX4, DEAD Box Protein 4, Vasa Homolog, VASA)
DDX4 belongs to the DEAD box helicase family or DDX4/VASA subfamily. DEAD box proteins are characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif and are putative RNA helicases. This protein has a glycine-rich N-terminus with multiple repeats of an RGG motif, which is believed to function in RNA binding. They have a role in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. The members of this family are involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila. It is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. It is required during gametogenesis in the adult and in the specification of the germ cell lineage during embryogenesis. This is conserved in invertebrate and vertebrate species.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceRabbit
Synthetic peptide corresponding to aa700-724 of DDX4.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Recognizes DDX4. Species Crossreactivity: chimpanzee, canine, equine, human, monkey, mouse. Species sequence homology: opossum, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Abe, K. et al. Mammalian Genome. 8:622-623 (1997). 2. Castrillion, D. et al. Proc. Nat. Acad. Sci. 97:9585-9590 (2000). 3. Chu, D. et al. Nature. 443:101-105 (2006).