Technical Data
D1660-27
DDX4 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 4, DEAD Box Protein 4, MGC111074, Probable ATP-dependent RNA Helicase DDX4, Vasa Homolog, VASA)
Description:
DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to the germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer suggesting its role in the icroRNA-mediated
RNA silencing (1).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG111C1025Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified recombinant fragment of human DDX4.
Purity:
Purified
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of total DDX4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1) Kotaja, N. et al. (2006) Proc Natl Acad Sci USA 103,2647-52. (2) Albamonte, M.S. et al. (2008) Hum Reprod 23,1895-901.