Technical Data
D1660-40B
DDX5 (DEAD (Asp-Glu-Ala-Asp) Box 5, DKFZp686J01190, G17P1, HLR1, HUMP68, p68)
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Technical Data |
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D1660-40B |
DDX5 (DEAD (Asp-Glu-Ala-Asp) Box 5, DKFZp686J01190, G17P1, HLR1, HUMP68, p68) |
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Description: DDX belongs to the family of DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, which are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:2500-1:10,000 Immunoprecipitation: 2-5ug/mg lysate Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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