Technical Data
D1660-40H
DDX5 (Probable ATP-dependent RNA Helicase DDX5, DEAD Box Protein 5, RNA Helicase p68, G17P1, HELR, HLR1)
Description:
DDX5, also known as p68 RNA helicase, is a 614aa, 68kD member of the DEAD box family of RNA helicases that contain the conserved Asp-Glu-Ala-Asp (DEAD) motif. DDX5 influences RNA transport, transcription, ribosome assembly, spliceosome function, RNA degradation, and the initiation of translation. DDX5 shares 87% aa identity with DDX-17 within the central region and the two are often expressed together. The C-terminal region used as an immunogen shares 96% and 99% aa identity with mouse and rat DDX5, respectively, but lies mainly outside the DDX-17 homology region.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Western Blot: 1:64,000 & 0.11-0.3ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-PMIGYPMPTGYSQ from C-terminus of human DDX5.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human DDX5. Species sequence homology: Canine, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.