Technical Data
D1661-05B
DDX23 (DDX-23, DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 23, PRPF28, PRPF-28, PRP28p Homolog, U5-100K, U5snRNP 100kD)
Description:
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
The epitope recognized maps to a region between residue 775 and the C-terminus (residue 820) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 using the numbering given in entry NP_004809.2 (GeneID 9416).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.1% sodium azide.
Specificity:
Species Crossreactivity: Human and mouse. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.