Technical Data
Media
Decarboxylase Broth Moeller (Powder)
D1830
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Beef Extract5.0
Bromcresol Purple0.01
Cresol Red0.005
Dextrose0.5
Peptic Digest of Animal Tissue5.0
Pyridoxal0.005
Total:10.5g/liter
When supplemented with appropriate substrates, used to differentiate gram negative enteric bacteria.

Moeller first described the use of an amino acid decarboxylase test to distinguish between various microorganisms. He further noted that the production of lysine, arginine, ornithine and glutamic acid decarboxylase helped to differentiate between gram-negative enteric bacteria. Ewing et al. concluded, after investigating the decarboxylase activity of 2937 cultures of the Enterobacteriaceae, that the Moeller formulation should be considered the standard medium for studying decarboxylate reactions. Bacteria which produce decarboxylase will produce an alkaline reaction (purple or violet). Decarboxylase negative bacteria will give an acidic reaction (yellow) due to the fermentation of dextrose. To insure proper reactions overlay medium with sterile mineral oil.


Directions per Liter: Dissolve 10.5grams per liter of distilled/ deionized (DDI) water, add 1% of L- or 2% of DL- lysine, arginine or ornithine as desired, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 10 minutes at 121C (15psi). Aseptically overlay inoculated broth tubes and controls with 4-5 mm of sterile mineral oil.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light beige, homogeneous, free flowing powder

Solubility: Reddish grey, clear, complete

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.