Significant expression of Dectin-2 has been reported on macrophages in the red pulp and marginal zones of the spleen, kupffer cells in the liver and alveolar macrophages in the lung. Peripheral blood monocytes express low levels of dectin-2 but transient up-regulation of expression has been demonstrated on monocytes at sites of inflammation.
The function of dectin-2 has not been fully determined but studies suggest a possible role in immune-surveillance.
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Flow Cytometry: 1:10-1:100; 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Dectin-2 (extracellular domain) Fc Fusion protein.|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.4. No preservative added.|
|Recognizes mouse Dectin-2, a type II transmembrane protein that is a member of the C-type lectin superfamily. In mouse, Dectin-2 is predominantly expressed on tissue macrophages and some dendritic cells. Does not stain brain microglia or meningeal macrophages.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Taylor, P.R. et al. (2005) Dectin-2 is predominantly myeloid restricted and exhibits unique activation-dependent expression on maturing inflammatory monocytes elicited in vivo. Eur J Immunol. 35:2163 - 2174. 2. Suram, S. et al. (2010) Pathways regulating cytosolic phospholipase A2 activation and eicosanoid production in macrophages by Candida albicans. J Biol Chem. 285: 30676-85. 3. Barrett, N.A. et al. (2011) Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes. J Exp Med. 208: 593-604. 4. Robinson, M. et al. (2009) Dectin-2 is a Syk-coupled pattern recognition receptor crucial for Th17 responses to fungal infection. J Exp Med. 206:2037-51. 5. McDonald, J.U. et al. (2012) Differential dependencies of monocytes and neutrophils on dectin-1, dectin-2 and complement for the recognition of fungal particles in inflammation. PLoS One. 7: e45781. 6. Ritter, M. et al. (2010) Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3 inflammasome and alters adaptive immune responses. Proc Natl Acad Sci U S A. 107: 20459-64. 7. Pugholm LH et al. (2015) In vitro Assay for Screening of Optimal Targets for Antigen-Delivery to Murine Dendritic Cells. Scand J Immunol. 82 (6): 498-505.|