		Technical Data
D1876-49D2	Dectin 2 (Dendritic Cell-associated C-type Lectin 2, DC-associated C-type Lectin 2, Dectin-2, C-type Lectin Domain Family 6 Member A, CLEC6A, C-type Lectin Superfamily Member 10, CLECSF10)

Description:
DC-associated C-type lectin-2 (Dectin-2), a type II transmembrane protein that is a member of the C-type lectin superfamily. In mouse, dectin-2 is predominantly expressed on tissue macrophages and some dendritic cells. Significant expression of Dectin-2 has been reported on macrophages in the red pulp and marginal zones of the spleen, kupffer cells in the liver and alveolar macrophages in the lung. Peripheral blood monocytes express low levels of dectin-2 but transient up-regulation of expression has been demonstrated on monocytes at sites of inflammation.
The function of Dectin-2 has not been fully determined but studies suggest a possible role in immune-surveillance.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies Murine X-Adsorbed:
I1904-50U1 IgG H&L, X-Adsorbed Murine (HRP) Pab GtxRt
I1904-74H IgG, X-Adsorbed Murine (HRP) Pab GtxRt
1904-74F IgG, Murine X-Adsorbed (FITC) Pab GtxRt
I1903-13C IgG, H&L, X-Adsorbed Murine (PE) Pab GtxRt
I1903-13N IgG, H&L, X-adsorbed Murine (PE) Pab GtxRt

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	10B2695	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Rat

Concentration:

~1mg/ml

Immunogen:

Dectin-2 (extracellular domain) Fc Fusion protein.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Specificity:

Recognizes mouse Does not stain brain microglia or meningeal macrophages.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Taylor, P.R. et al. (2005) Dectin-2 is predominantly myeloid restricted and exhibits unique activation-dependent expression on maturing inflammatory monocytes elicited in vivo. Eur J Immunol.35:2163 - 2174.