Technical Data
DAD1 (Dolichyl-diphosphooligosaccharide-protein Glycosyltransferase Subunit DAD1, Oligosaccharyl Transferase Subunit DAD1, Defender Against Cell Death 1, DAD-1)
Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis.DD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lackingDD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression ofDD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted thatDD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HepG2 cell lysates.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to human DAD1.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes human DAD1. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nakashima T, Sekiguchi T, Kuraoka A, et al. Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells. Mol. Cell Biol. 1993; 13:6367-74. 2. Stellar H. Mechanisms and genes of cellular suicide. Science 1995; 267:1445-9. 3. Gilmore R and Kelleher DJ. DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase. Proc. Natl. Acad. Sci. USA 1997; 94:4994-9. 4. Sanjay A, Fu J, and Kreibich G. DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex. J. Biol. Chem. 1998; 273:26094-9. 5. Hong NA, Flannery M, Hsieh SN, et al. Mice lacking Dad1, the defender against apoptotic death-1, express abnormal N-linked glycoproteins and undergo increased embryonic apoptosis. Dev. Biol. 2000; 220:76-84. 6. Brewster JL, Martin SL, Toms J, et al. Deletion of DAD1 in mice induces an apoptosis-associated embryonic death. Genesis 2000; 26:271-8. 7. Tanaka K, Kondoh N, Shuda M, et al. Enhanced expression of mRNAs of antisecretory factor-1, gp96, DAD1 and CDC34 in human hepatocellular carcinomas. Biochim. Biophys. Acta. 2001; 1536:1-12.