Technical Data
Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes. 3H2072 recognizes alpha defensins (human neutrophil peptides). Binding to defensin 4 has not been tested.

Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: (frozen and paraffin) 1:1000, Requires protein digestion pre-treatment of paraffin sections. e.g. trypsin or pronase.
Flow Cytometry: Requires membrane permeabilization.
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab)2, X-Adsorbed (HRP) Pab RbxMo
F(ab')2 Rabbit anti mouse IgG: PE

Recommended Positive Control Tissue:
Neutrophils, HL-60 cell line

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

100ug4C (-20C Glycerol)Blue IceHumanMouse
Purified IgG
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and 0.5% BSA.
Recognizes human defensin, expressed by mature neutrophils.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mller, C.A. et al. (2002) Human alpha - defensins HNPs-1, -2 and -3 in Renal Cell Carcinoma. Am. J. Path. 160: 1311-1324