Technical Data
D1950
Defensin
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Technical Data |
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D1950 |
Defensin |
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Description: Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes. 3H2072 recognizes alpha defensins (human neutrophil peptides). Binding to defensin 4 has not been tested. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: (frozen and paraffin) 1:1000, Requires protein digestion pre-treatment of paraffin sections. e.g. trypsin or pronase. Flow Cytometry: Requires membrane permeabilization. Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo F(ab')2 Rabbit anti mouse IgG: PE Recommended Positive Control Tissue: Neutrophils, HL-60 cell line Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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