Technical Data
Media
Defined Nutrient Powder (DNP) #1 (Powder)
D1955
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Ammonium Sulfate429
Calcium Chloride5.6
Ferric Chloride*6H2O2.7
Magnesium Chloride95.4
Potassium Chloride100
Sodium Chloride1000
Zinc Sulfate•7H2O0.8
Manganese Sulfate•H2O0.3
Amino Acids:
L-Alanine300
L-Arginine HCl1270
L-Asparagine1000
L-Aspartic acid20
L-Cystine•Na2•H2O302
L-Glutamic acid20
L-Glutamine2520
Glycine500
L-Histidine310
L-Isoleucine520
L-Leucine520
L-Lysine•HCI725
L-Methionine150
L-Phenylalanine320
L-Proline1000
L-Serine30
L-Threonine480
L-Tryptophan100
L-Tyrosine•Na2•2H2O519
L-Valine460
Vitamins:
p-aminobenzoic acid1
Biotin0.5
D-Pantothenic Acid•Ca25
Choline Chloride25
i-Inositol25
Nicotinamide5
Pyridoxal•HCI5
Thiamine•HCI5
Other:
Uracil25
Adenine25
Total:12.85g/L



Directions per Liter: Dissolve 12.95g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Endotoxin: ~10EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.