Technical Data

Media

Defined Nutrient Powder (DNP) #1 (Powder)

D1955

Storage RT Shipping RT

Components shown as mg/liter


Inorganic Salts:
Ammonium Sulfate	429
Calcium Chloride	5.6
Ferric Chloride*6H2O	2.7
Magnesium Chloride	95.4
Potassium Chloride	100
Sodium Chloride	1000
Zinc Sulfate•7H2O	0.8
Manganese Sulfate•H2O	0.3

Amino Acids:
L-Alanine	300
L-Arginine HCl	1270
L-Asparagine	1000
L-Aspartic acid	20
L-Cystine•Na2•H2O	302
L-Glutamic acid	20
L-Glutamine	2520
Glycine	500
L-Histidine	310
L-Isoleucine	520
L-Leucine	520
L-Lysine•HCI	725
L-Methionine	150
L-Phenylalanine	320
L-Proline	1000
L-Serine	30
L-Threonine	480
L-Tryptophan	100
L-Tyrosine•Na2•2H2O	519
L-Valine	460

Vitamins:
p-aminobenzoic acid	1
Biotin	0.5
D-Pantothenic Acid•Ca	25
Choline Chloride	25
i-Inositol	25
Nicotinamide	5
Pyridoxal•HCI	5
Thiamine•HCI	5

Other:
Uracil	25
Adenine	25

Total:	12.85g/L

Directions per Liter: Dissolve 12.95g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Endotoxin: ~10EU/mg

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.