		Technical Data
D2675-01A	DAZL, phosphorylated (Tyr276) (Deleted in azoospermia-like, DAZ-like autosomal, Deleted in azoospermia-like 1, DAZ homolog, SPGY-like-autosomal, DAZL, DAZH, DAZL1, DAZLA, SPGYLA)

Description:
The DAZ (Deleted in AZoospermia) family are potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. DAZL is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa.

Applications:
Suitable for use in ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr276 of human DAZL (KLH).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human DAZL when phosphorylated at Tyr276.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Zerbetto,I., Fertil. Steril. 90(6), 2264-2268 (2008). 2. Stefanidis,K., Fertil. Steril. 90(3), 798-804 (2008).