Technical Data
D2675-02C
Deleted in Azoospermia-Like (DAZL, DAZL1, DAZ Homolog, DAZH, DAZ-like Autosomal, DAZLA, MGC26406, SPGY-like-autosomal, SPGYLA)
Description:
DAZL is involved in mitosis and meiosis of human germ cells. The DZL gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of diverse mammalian species. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In humans, DZL is expressed in primordial germ cells, adult testis, and the ovary. It plays a central role during spermatogenesis. Defects in DZL may cause infertility due to severe oligozoospermia (az) and non obstructive azoospermia. The concentrations of DZL transcripts were found to be lower in men with spermatogenic failure. Drosophila and mouse mutants that have lost DZL homologous gene function found to be azoospermic, just as with human azoospermia.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.3ug/ml
ELISA: >1:64,000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Ovary lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-GNGPQKKSVDR, from the internal region of the protein sequence according to NP_001342.2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human DAZL. Species sequence homology: mouse, rat, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cauffman G, Van de Velde H, Liebaers I, Van Steirteghem A. DAZL expression in human oocytes, preimplantation embryos and embryonic stem cells. Mol Hum Reprod. 2005 Jun;11(6):405-411. Epub 2005 May 6. PMID: 15879466.