Technical Data
Media
D/E Neutralizing Agar (Powder)
D2700
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Yeast Extract2.5
Dextrose10.0
Casein Digest Peptone5.0
Lecithin7.0
Sodium Thioglycollate1.0
Sodium Thiosulfate6.0
Sodium Bisulfite2.5
Polysorbate 805.0
Bromocresol Purple0.02
Agar15.0
Total:54g/liter
Recovery of bacteria from surfaces cleansed with disinfectants.

Description:
D/E Neutralizing Agar is capable of neutralizing a broad spectrum of antiseptic and disinfectant chemicals including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde and glutaraldehyde. It can determine the bactericidal capability of disinfectants and therefore is well suited for environmental sampling.

Storage:
Store the sealed bottle containing the dehydrated medium at 2 to 30.0C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light bluish grey color.


Directions: 1. Dissolve 54grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi) Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Blueish-grey, homogeneous, lumpy powder.

Solubility: Purple, opaque, complete.

pH (25C): 7.60.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.