Technical Data
DLL1 (Delta-like Protein 1, Drosophila Delta Homolog 1, Delta1, H-Delta-1, UNQ146/PRO172)
The Notch signaling pathway is an evolutionarily conserved pathway in multi­cellular organisms, which is vital for cell­cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T­cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta­like protein1(DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM­family metalloproteases and the gamma­secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL­NICD­Mastermind complex cascade (canonipathway),NF­kappaB­NICD and CSL­NICD­Deltex complex signaling cascades (non­canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non­canonical pathway promotes differentiation.DLL1 is widely expressed, and acts as a mediator of cell fate decisions during hematopoiesis, and may play a role in cell­to­cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signaling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB).

Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 1x10e6 cells in 100ul
Immunohistochemistry: Frozen sections. Suitability for use with paraffin sections has not been determined.
Optimal dilutions to be determined by the researcher.

P3U1 myeloma cells with spleen cells from Armenian hamsters.

Positive Control Tissue:
Mouse spleen

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgGHMD1-5Affinity Purified
100ug-20°CBlue IceMouseHamster
DLL1­expressing CHO cells.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS containing 0.09% sodium azide.
Recognizes Delta-like protein1 (DLL1). Species Crossreactivity: Rat. Weakly recognizes human DLL1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Moriyama, Y. et al. (2008) Delta­like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. Int. Immunol. 20: 763­773. 2. Sekine, C. et al. (2009) Differential regulation of splenic CD8­ dendritic cells and marginal zone B cells by Notch ligands. Int. Immunol. 21: 295­301.