Technical Data
D2727-75D
DLL1 (Delta-like Protein 1, Drosophila Delta Homolog 1, Delta1, H-Delta-1, UNQ146/PRO172)
Description:
The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. DLL1 is widely expressed, and acts as a mediator of cell fate decisions during haematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signalling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB).

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 1x10e6 cells in 100ul
Immunohistochemistry: Frozen, Paraffin
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3U1 myeloma cells with spleen cells from Armenian hamsters.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12G26Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseHamster
Concentration:
~1mg/ml
Immunogen:
DLL1-expressing CHO cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1mg/ml BSA, 0.9% sodium azide.
Specificity:
Recognizes Delta-like protein 1 (DLL1). Species Crossreactivity: Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Moriyama, Y. et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. Int. Immunol. 20: 763-773. 2. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands. Int. Immunol. 21: 295-301.