Technical Data
D2727-85A
DLL3 (Delta-like Protein 3, Drosophila Delta Homolog 3, Delta3)
Description:
Delta-like protein 3 is a ligand for the Notch signaling pathway. It inhibits primary neurogenesis. DLL3 plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Defects in DLL3 are the cause of Spondylocostal dysostosis autosomal recessive type 1 (SCDO1). Mutations in DLL3 gene cause truncal shortening relative to their limbs, which leads to abdominal protrusion, abnormal spinal curvature and sometimes a plagiocephaly-torticollis sequence. It may be required to divert neurons along a specific differentiation pathway.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 3-5ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse embryo brain and T98G (human) cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa100 to 150 of human DLL3.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human DLL3. Species Crossreactivity: monkey, mouse. Species sequence homology: bovine, canine, equine, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bulman, MP. et al. Nat. Genet. 24:438-441 (2000). 2. Turnpenny, PD. et al. J. Med. Genet. 40:333-339 (2003). 3. Zhao, X. et al. Dev. Cell. 17:210-221 (2009).