Technical Data
D2728-01D
Delta 4 (Delta-like Protein 4, DLL4, DLL-4, Delta Ligand 4 Precursor, Drosophila Delta Homolog 4, hdelta2, MGC126344, Notch Ligand DLL4 Precursor)
Description:
DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 5-15ug/ml
ELISA: 1:3000-1:15000
Western Blot: 1:300-1:1500. Detects a band at ~74kD in pancreatic cell lysates.
Optimal dilutions to be determined by the researcher.

Positive Control: human ovary

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to the internal region of human DLL4.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:
Recognizes human Delta-like ligand 4 (DLL4). Species Sequence Homology: mouse, rat and chimpanzee.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Thurston, G. et al. (2007) The Delta paradox: DLL4 blockade leads to more tumour vessels but less tumour growth. Nat. Rev. Cancer. 7: 327-331.