Technical Data
D2728-01E
Delta 4 (Delta-like Protein 4, DLL4, DLL-4, Drosophila Delta Homolog 4)
Description:
Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis. Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine­rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. Human and mouse DLL4 share 86% aa sequence identity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a11C12Affinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceMouseRat
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Mouse myeloma cell line, NS0-derived recombinant mouse DLL4, Ser28-Pro525 (Accession # Q9JI71)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
Specificity:
Recognizes mouse DLL4. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313. 2. Iso, T. et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543. 3. Walker, L. et al. (2001) Stem Cells 19:543. 4.Baron, M. (2002) Semin. Cell Dev. Biol. 14:113. 5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.