Technical Data
DLL4 (Delta-like Protein 4, Drosophila Delta Homolog 4, Delta4, UNQ1895/PRO4341)
The Notch signalling pathway is an evolutionarily conserved pathway in multi­cellular organisms, which is vital for cell­cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T­cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1(CD339), Jagged2, Delta like-1(DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM­family metalloproteases and gamma­secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL­NICD­Mastermind complex cascade (canonical pathway), or NF­kappaB­NICD and CSL­NICD­ Deltex complex signalling cascades (non­canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non­canonical pathway promotes differentiation. DLL4 is expressed by vascular endothelium, and plays a vital role in embryonic vascular development. DLL4 signalling has been shown to play a role in the angiogenesis of clear­cell renal tumors, and pancreatic, bladder and colonic cancer. Studies have shown that DLL4 expression in endothelium cells, can be up­regulated by vascular endothelial growth factor (VEGF) and basic­FGF, and by HIF1 alpha, and that blockade of DLL4 inhibits tumor growth by promoting non­productive angiogenesis.
Clone HMD4­2 has been shown to block mouse Notch1­Fc and Notch4­Fc binding to mouse DLL4:CHO cells, and to inhibit subcutaneous tumor growth in mouse tumor models using the murine lung carcinoma cell line KLN205(3).

Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 1x10e6 cells in 100ul
Immunohistochemistry: Frozen, Paraffin
Optimal dilutions to be determined by the researcher.

P3U1 myeloma cells with spleen cells from Armenian hamsters.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG12G28Affinity Purified
100ug-20°CBlue IceMouseHamster
Recombinant corresponding to mouse DLL4.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 6.0, 0.09% sodium azide.
Recognizes mouse DLL4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Moriyama, Y. et al. (2008) Delta­like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. Int. Immunol. 20: 763­773. 2. Sekine, C. et al. (2009) Differential regulation of splenic CD8­ dendritic cells and marginal zone B cells by Notch ligands. Int. Immunol. 21: 295­301. 3. Yamanda, S. et al. (2009) Role of ephrinB2 in nonproductive angiogenesis induced by Delta­like 4 blockade. Blood. 113: 3631­3639. 1. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex. Nat. Rev. Mol. Cell Biol. 7: 678­689. 2. Iso, T. et al. (2003) Notch signaling in vascular development. Arterioscler. Thromb. Vasc. Biol. 23: 543­553. 3. Hu, X. et al. (2008) Integrated regulation of Toll­like receptor responses by Notch and interferon­gamma pathways. Immunity. 29: 691­703 4. Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity. Immunol. Rev. 182: 215­227.