Technical Data
D2728-01K
DLL4 (Delta-like Protein 4, Drosophila Delta Homolog 4, Delta4, UNQ1895/PRO4341)
Description:
The Notch signalling pathway is an evolutionarily conserved pathway in multi­cellular organisms, which is vital for cell­cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T­cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1(CD339), Jagged2, Delta like-1(DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM­family metalloproteases and gamma­secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL­NICD­Mastermind complex cascade (canonical pathway), or NF­kappaB­NICD and CSL­NICD­ Deltex complex signalling cascades (non­canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non­canonical pathway promotes differentiation. DLL4 is expressed by vascular endothelium, and plays a vital role in embryonic vascular development. DLL4 signalling has been shown to play a role in the angiogenesis of clear­cell renal tumors, and pancreatic, bladder and colonic cancer. Studies have shown that DLL4 expression in endothelium cells, can be up­regulated by vascular endothelial growth factor (VEGF) and basic­FGF, and by HIF1 alpha, and that blockade of DLL4 inhibits tumor growth by promoting non­productive angiogenesis.
Clone HMD4­2 has been shown to block mouse Notch1­Fc and Notch4­Fc binding to mouse DLL4:CHO cells, and to inhibit subcutaneous tumor growth in mouse tumor models using the murine lung carcinoma cell line KLN205(3).

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 1x10e6 cells in 100ul
Immunohistochemistry: Frozen, Paraffin
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3U1 myeloma cells with spleen cells from Armenian hamsters.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12G28Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseHamster
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to mouse DLL4.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 6.0, 0.09% sodium azide.
Specificity:
Recognizes mouse DLL4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Moriyama, Y. et al. (2008) Delta­like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. Int. Immunol. 20: 763­773. 2. Sekine, C. et al. (2009) Differential regulation of splenic CD8­ dendritic cells and marginal zone B cells by Notch ligands. Int. Immunol. 21: 295­301. 3. Yamanda, S. et al. (2009) Role of ephrinB2 in nonproductive angiogenesis induced by Delta­like 4 blockade. Blood. 113: 3631­3639. 1. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex. Nat. Rev. Mol. Cell Biol. 7: 678­689. 2. Iso, T. et al. (2003) Notch signaling in vascular development. Arterioscler. Thromb. Vasc. Biol. 23: 543­553. 3. Hu, X. et al. (2008) Integrated regulation of Toll­like receptor responses by Notch and interferon­gamma pathways. Immunity. 29: 691­703 4. Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity. Immunol. Rev. 182: 215­227.