Technical Data
D2810-85
Dengue Virus
Description:
The dengue virus is responsible for the tropical and sub-tropical diseases, dengue (DF) and dengue haemorrhagic fever (DHF), transmitted to individuals through the bite of the Aedes mosquito. The global distribution of dengue is expanding and comparable to that of malaria, with symptoms ranging from a mild viral syndrome of high fever, rash, headache, fatigue and muscle and joint pain, to the more severe and sometimes fatal DHF. Each dengue virus serotype is antigenically distinct, such that infection only provides an individual with immunity to the causative serotype, with the possibility of further infection by a different serotype or even multiple serotypes.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. sodium citrate buffer, pH 6.0 is recommended.
Western Blot (reducing): Recognizes E/envelope at 61kD.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a10B2710Affinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Dengue Virus.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity:
Recognizes all four dengue virus serotypes (DEN-1, DEN-2, DEN-3 and DEN-4) of the genus Flavivirus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.