Technical Data
D3165-02
11-dATP (Biotin) (Biotin-11-dATP)
10ul
Molecular Biology Storage: -20°CShipping: Blue Ice
Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine.

Elemental Analysis:
C: ~40%, H: ~5%, N: ~12%, O: ~27%, P: ~10%, S: ~3%

Spectroscopic Properties:
max 280nm; 12,700

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Formula:
C30H45N8O15P3S

Molecular Weight:
882.71
Purity: ~95%
Concentration: ~1mM
Form: Supplied as a liquid in 10mM Tris, pH 7.5. Labeled with Biotin.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
[1. Zammatteo et al. (2005) Unambiguous identification of the expressed MAGE-A genes on a DNA microarray. Clin. Chem. 51(12):2420. [2. Dauwerse et al. (1999) Two-colour FISH detection of the inv(16) in interphase nuclei of patients with acute myeloid leukaemia. Br. J. Haematol. 106(1):111. 3. Vorwerk et al. (2008) Microfluidic-based enzymatic on-chip labeling of miRNAs. N. Biotechnol. 25(2):142. 4. Beier et al. (2008) Microfluidic primer extension assay. Methods Mol Biol 822:143. 5. Vankoningsloo et al. (2008) Gene expression silencing with ’specific’ small interfering RNA goes beyond specificity - a study of key parameters to take into account in the onset of small interfering RNA off-target effects. FEBS J. 275(11):2738.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.